Part:BBa_K411100:Design

Eukaryote Initiation Factor 4A (eIF4A) from Mus Musculus

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 240
  Illegal BglII site found at 536
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 972
  Illegal BsaI.rc site found at 638
  

Design Notes

Mutated two PstI cutting sites at positions 223 and 391 from ctgcag to ctccag.

Source

[http://www.ncbi.nlm.nih.gov/nuccore/NM_144958 eIF4A] from Mus Musculus